A simple radioassay for angiotensin-converting enzyme.

نویسندگان

  • J W Ryan
  • A Chung
  • C Ammons
  • M L Carlton
چکیده

Angiotensin-converting enzyme can be measured by the rate of release of 3H-labelled hippurate from p-[3H]benzoylglycylglycylglycine. The product is separable from the substrate by extraction of acidified reaction mixtures with ethyl acetate. Assay results for human serum angiotensin-converting enzyme can be obtained within 1.5 h of receipt of serum samples. Within the limits tested, the assay appears to be specific. However, interference by hitherto unrecognized enzymes of abnormal sera must be ruled out.

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عنوان ژورنال:
  • The Biochemical journal

دوره 167 2  شماره 

صفحات  -

تاریخ انتشار 1977